RNA-protein complexes are fundamental to numerous cellular processes, including translation and gene regulation. The high-resolution structural determination of RNA-protein complexes is essential to understanding their functions. Therefore, computational methods capable of identifying native-like RNA-protein structures are needed. To address this challenge, we have developed DRPScore, a deep-learning-based approach for identifying native-like RNA-protein structures.
DRPScore has been tested on representative sets of RNA-protein complexes that exhibit various degrees of binding-induced conformation changes. These range from fully rigid docking (bound-bound) to fully flexible docking (unbound-unbound). Out of the top 20 predictions, DRPScore selects native-like structures with a success rate of 91.67% for bound RNA-protein complexes and 56.14% for unbound complexes. DRPScore consistently outperforms existing methods, achieving a 10.53-15.79% improvement, even in the most challenging unbound cases. Furthermore, DRPScore significantly enhances the accuracy of native interface interaction predictions. DRPScore is expected to be widely useful in modeling and designing RNA-protein complexes.
Biological processes such as transcription, repair, and regulation require interactions between DNA and proteins. To unravel their functions, it is imperative to determine the high-resolution structures of DNA-protein complexes. However, experimental methods for this purpose are costly and technically demanding. Consequently, there is an urgent need for computational techniques to identify the structures of DNA-protein complexes. Despite technological advancements, accurately identifying DNA-protein complexes through computational methods still poses a challenge.
Our team has developed a cutting-edge deep-learning approach called DDPScore that assesses DNA-protein complex structures. DDPScore utilizes a 4D convolutional neural network to overcome limited training data. This approach effectively captures local and global features while comprehensively considering the conformational changes arising from the flexibility during the DNA-protein docking process. DDPScore consistently outperformed the available methods in comprehensive DNA-protein complex docking evaluations, even for the flexible docking challenges. DDPScore has a wide range of applications in predicting and designing structures of DNA-protein complexes.
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Institute of Biophysics and Department of Physics, Central China Normal University, Wuhan 430079, China.